|Categories||Building Blocks Organics|
|Harmonised Tariff Code||29212900 EXP 2921290090 IMP|
Buy Online 1.5-Diaminopentane : In the growing era of the bioeconomy, the five-carbon compound 1,5-diaminopentane is gaining increasing interest as a platform chemical, especially as an innovative building block for bio-based polymers. The vital interest in bio-based supplies of 1,5-diaminopentane strongly stimulated research into the development of artificial production strains. Based on the latest knowledge of the pathways and reactions involved in the microbial metabolism of 1,5-diaminopentane, the review covers novel systems metabolic engineering approaches to the hyperproductive cellular factories of Corynebacterium glutamicum or Escherichia coli. This is integrated across the entire value chain from renewable feedstocks to 1,5-diaminopentane to innovative biopolymers incorporating bioprocess engineering considerations for economic delivery.
Macrophage-like RAW 264 and H35 hepatoma cells grown under serum-free conditions exported putrescine and an unidentified diamine into the culture medium. Unlike putrescine, the unknown compound could only be detected extracellularly. Analysis of dansylated polyamine standards and mass spectroscopy confirmed that the unknown compound was cadaverine (1,5-diaminopentane). Cells were free of mycoplasmas as evidenced by a negative result using a probe specific for prokaryotic rRNA. After prophylactic treatment with two different mycoplasmacidal agents, the cells continued to export cadaverine. Attempts to “infect” a non-cadaverine-exporting cell line with culture medium and cell-free lysates proved unsuccessful and demonstrated that cadaverine was in fact a bona fide product of these mammalian cells. Cadaverine export by RAW 264 and H35 cells was stimulated by lipopolysaccharide and insulin. However, administration of exogenous ornithine caused cadaverine export to decrease significantly with a concomitant increase in putrescine export. alpha-difluoromethylornithine, a selective inhibitor of ornithine decarboxylase, inhibited the export of both cadaverine and putrescine. When cells were labeled with [3H]lysine, the vast majority of radioactivity recovered in exported polyamines was found in cadaverine. Cumulative data suggest that the formation of cadaverine may be due to the action of intracellular ornithine decarboxylase on lysine to form cadaverine, which is then removed from the cell with a high degree of efficiency.